Medpedia

• Celiac Disease
• Gluten

Inclusion Criteria:
-  HLA-DQ2+ celiacs and HLA-DQ2+ persons who have been on a gluten free diet for at
least 4 weeks.
Exclusion Criteria:Pregnancy
-  Other severe disease
-  Refusal to participate
-  HLA-DQ2 negativity

• Dietary Supplement: Gluten challenge.
  The study population consists of 50 HLA-DQ2+ patients on a gluten free diet, both celiacs and uncertain celiacs. The patients will undergo a oral gluten challenge with four slices of bread daily for three days. Before challenge blood samples will be drawn for tetramer staining of gluten-specific T-cells. The analysis is done at the Institute of Immunology at Rikshospitalet, using FACS analysis. Small intestinal biopsies will be examined for morhological changes and inflammatory gene expression. These procedures with blood samples and biopsies will be repeated after gluten challenge. Registration for clinical symptoms and personality traits will be done by standardised and validated forms. A dietist will perform an interview of the patients.

• HLA-DQ2-tetramer response after gluten challenge
  6 days.
  Safety Issue: No
• Mucosal responses to 3 day gluten challenge.
  4 days.
  Safety Issue: No

• Department of Medicine, Endoscopy Unit, Rikshospitalet
  Oslo, 0027, Norway
  Recruiting
  Knut EA Lundin, MD, PhD

  0

  knut.lundin@rikshospitalet.no

  Investigators

  • Knut EA Lundin, MD, PhD
    Principal Investigator

• Rikshospitalet HF
  Lead Sponsor
• Norway: Royal Norwegian Ministry of Health and Care Services

• Ráki M, Fallang LE, Brottveit M, Bergseng E, Quarsten H, Lundin KE, Sollid LM. Tetramer visualization of gut-homing gluten-specific T cells in the peripheral blood of celiac disease patients. Proc Natl Acad Sci U S A. 2007 Feb 20;104(8):2831-6. Epub 2007 Feb 16.